Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A comprehensive proteomics-based interaction screen that links DYRK1A to RNF169 and to the DNA damage response.

Roewenstrunk J, Di Vona C, Chen J, Borras E, Dong C, Arato K, Sabido E, Huen MSY, de la Luna S

Dysregulation of the DYRK1A protein kinase has been associated with human disease. On the one hand, its overexpression in trisomy 21 has been linked to certain pathological traits of Down syndrome, while on the other, inactivating mutations in just one allele are responsible for a distinct yet rare clinical syndrome, DYRK1A haploinsufficiency. Moreover, altered expression of this kinase may also ... [more]

Sci Rep Apr. 12, 2019; 9(1);6014 [Pubmed: 30979931]

Throughput

  • High Throughput

Additional Notes

  • hit proteins identified in IP of bait DYRK1A epitope tagged with either of 2 C-terminal tags or an N-terminal tag

Curated By

  • BioGRID