BAIT

CDK6

MCPH12, PLSTIRE
cyclin-dependent kinase 6
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

SUMO1 modification stabilizes CDK6 protein and drives the cell cycle and glioblastoma progression.

Bellail AC, Olson JJ, Hao C

Ubiquitination governs oscillation of cyclin-dependent kinase (CDK) activity through a periodic degradation of cyclins for orderly cell cycle progression; however, the mechanism that maintains the constant CDK protein levels throughout the cell cycle remains unclear. Here we show that CDK6 is modified by small ubiquitin-like modifier-1 (SUMO1) in glioblastoma, and that CDK6 SUMOylation stabilizes the protein and drives the cell ... [more]

Nat Commun Jun. 23, 2014; 5();4234 [Pubmed: 24953629]

Throughput

  • Low Throughput

Curated By

  • BioGRID