ACTN1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GRM5
Gene Ontology Biological Process
- G-protein coupled glutamate receptor signaling pathway [IMP, ISO, ISS]
- activation of MAPKKK activity [IDA]
- cognition [ISO]
- desensitization of G-protein coupled receptor protein signaling pathway [IDA]
- learning [ISO]
- locomotory behavior [ISO]
- negative regulation of locomotion [IDA]
- phosphatidylinositol-mediated signaling [TAS]
- phospholipase C-activating G-protein coupled glutamate receptor signaling pathway [TAS]
- positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway [TAS]
- positive regulation of long-term neuronal synaptic plasticity [IDA]
- protein kinase C-activating G-protein coupled receptor signaling pathway [IDA, TAS]
- protein phosphorylation [IDA]
- regulation of long-term neuronal synaptic plasticity [ISO]
- regulation of synaptic transmission, glutamatergic [IBA]
- regulation of transcription, DNA-templated [IDA]
- synaptic transmission [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Actin-binding protein alpha-actinin-1 interacts with the metabotropic glutamate receptor type 5b and modulates the cell surface expression and function of the receptor.
Receptors for neurotransmitters require scaffolding proteins for membrane microdomain targeting and for regulating receptor function. Using a yeast two-hybrid screen, alpha-actinin-1, a major F-actin cross-linking protein, was identified as a binding partner for the C-terminal domain of metabotropic glutamate receptor type 5b (mGlu(5b) receptor). Co-expression, co-immunoprecipitation, and pull-down experiments showed a close and specific interaction between mGlu(5b) receptor and alpha-actinin-1 ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID