BAIT

PGI1

CDC30, glucose-6-phosphate isomerase, L000001410, L000001409, YBR196C
Glycolytic enzyme phosphoglucose isomerase; catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
GO Process (3)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

STHA

ECK3954, JW5551, sth, udhA, b3962
pyridine nucleotide transhydrogenase, soluble
GO Process (0)
GO Function (0)
GO Component (0)
Escherichia coli (K12/MG1655)

Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Publication

Glucose utilization of strains lacking PGI1 and expressing a transhydrogenase suggests differences in the pentose phosphate capacity among Saccharomyces cerevisiae strains.

Heux S, Cadiere A, Dequin S

Saccharomyces cerevisiae strains lacking phosphoglucose isomerase (pgi1) cannot use the pentose phosphate (PP) pathway to oxidize glucose, which has been explained by the lack of mechanism for reoxidation of the NADPH surplus. Consistent with this, the defective growth on glucose of a ENYpgi1 strain can be partially restored by expressing the Escherichia coli transhydrogenase udhA. In this work it was ... [more]

FEMS Yeast Res. Mar. 01, 2008; 8(2);217-24 [Pubmed: 18036177]

Throughput

  • Low Throughput

Ontology Terms

  • metabolism and growth (APO:0000094)

Additional Notes

  • Expression of the E. coli transhydrogenase Udha/sthA can partially restore the growth of the ENYpgi1 yeast mutant on glucose.
  • S. cerevisiae does not have a functional ortholog of the E. coli transhydrogenase UdhA/sthA. This was not a cross-species complementation experiment.

Curated By

  • BioGRID