BAIT

PARP1

ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PPOL, pADPRT-1, RP11-125A15.2
poly (ADP-ribose) polymerase 1
Homo sapiens
PREY

NEDD8

NEDD-8
neural precursor cell expressed, developmentally down-regulated 8
UPS Project
Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Unanchored tri-NEDD8 inhibits PARP-1 to protect from oxidative stress-induced cell death.

Keuss MJ, Hjerpe R, Hsia O, Gourlay R, Burchmore R, Trost M, Kurz T

NEDD8 is a ubiquitin-like protein that activates cullin-RING E3 ubiquitin ligases (CRLs). Here, we identify a novel role for NEDD8 in regulating the activity of poly(ADP-ribose) polymerase 1 (PARP-1) in response to oxidative stress. We show that treatment of cells with H2O2 results in the accumulation of NEDD8 chains, likely by directly inhibiting the deneddylase NEDP1. One chain type, an ... [more]

EMBO J. Dec. 15, 2018; 38(6); [Pubmed: 30804002]

Throughput

  • Low Throughput

Additional Notes

  • assayed using GST (glutathione S-transferase) pull-down experiments

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PARP1 NEDD8
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID