An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Selective lowering of synapsins induced by oligomeric ?-synuclein exacerbates memory deficits.

Larson ME, Greimel SJ, Amar F, LaCroix M, Boyle G, Sherman MA, Schley H, Miel C, Schneider JA, Kayed R, Benfenati F, Lee MK, Bennett DA, Lesne SE

Mounting evidence indicates that soluble oligomeric forms of amyloid proteins linked to neurodegenerative disorders, such as amyloid-? (A?), tau, or ?-synuclein (?Syn) might be the major deleterious species for neuronal function in these diseases. Here, we found an abnormal accumulation of oligomeric ?Syn species in AD brains by custom ELISA, size-exclusion chromatography, and nondenaturing/denaturing immunoblotting techniques. Importantly, the abundance of ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 06, 2016; 114(23);E4648-E4657 [Pubmed: 28533388]

Throughput

Low Throughput

Additional Notes

Syn1 aggregates

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SYN1 SYN1	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Larson ME (2017)	Low	-	BioGRID	2639265
SYN1 SYN1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Stelzl U (2005)	High	2	BioGRID	-

Curated By

BioGRID

Interaction Summary

SYN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [TAS]protein binding [IPI]protein kinase binding [ISS]transporter activity [TAS]

Gene Ontology Cellular Component