BAIT

SLY1

L000001923, YDR189W
Hydrophilic protein involved in ER/Golgi vesicle trafficking; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex
Saccharomyces cerevisiae (S288c)
PREY

PRE1

proteasome core particle subunit beta 4, L000001483, YER012W
Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

SM-protein-controlled ER-associated degradation discriminates between different SNAREs.

Braun S, Jentsch S

Endoplasmic reticulum (ER)-associated degradation (ERAD) is a specialized activity of the ubiquitin-proteasome system that is involved in clearing the ER of aberrant proteins and regulating the levels of specific ER-resident proteins. Here we show that the yeast ER-SNARE Ufe1, a syntaxin (Qa-SNARE) involved in ER membrane fusion and retrograde transport from the Golgi to the ER, is prone to degradation ... [more]

EMBO Rep. Dec. 01, 2007; 8(12);1176-82 [Pubmed: 18007658]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)
  • phenotype: heat sensitivity (APO:0000147)

Additional Notes

  • The sly1-1 pre1-1 double mutant is inviable at elevated temperatures at which the single mutants can grow. Each single mutant also shows a temperature-sensitive phenotype of slow growth.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SLY1 PRE1
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
426606

Curated By

  • BioGRID