BASP1
Gene Ontology Biological Process
Gene Ontology Molecular Function
DNM1
Gene Ontology Biological Process
- G-protein coupled receptor internalization [IMP]
- adult locomotory behavior [ISO]
- endocytosis [ISO]
- endosome organization [ISO]
- positive regulation of synaptic vesicle recycling [IMP]
- protein tetramerization [ISO]
- receptor internalization [IMP]
- receptor-mediated endocytosis [IMP, ISO]
- sensory perception of sound [ISO]
- synaptic transmission, GABAergic [ISO]
- toxin transport [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Interaction of dynamin I with NAP-22, a neuronal protein enriched in the presynaptic region.
Neurons have well-developed membrane microdomains called "rafts" that are recovered as a detergent-resistant low-density membrane microdomain fraction (DRM). NAP-22 is one of the major protein components of neuronal DRM and localizes in the presynaptic region. In order to know the role of NAP-22 in the synaptic transmission, NAP-22 binding proteins in the cytosol were searched with an affinity screening with ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| BASP1 DNM1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| DNM1 BASP1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID