BAIT

MDK

ARAP, MK, NEGF2
midkine (neurite growth-promoting factor 2)
GO Process (6)
GO Function (2)
GO Component (1)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Midkine binds to anaplastic lymphoma kinase (ALK) and acts as a growth factor for different cell types.

Stoica GE, Kuo A, Powers C, Bowden ET, Sale EB, Riegel AT, Wellstein A

Midkine (MK) is a developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN). To investigate the potential role of MK in tumor growth, we expressed MK in human SW-13 cells and studied receptor binding, signal transduction, and activity of MK. The MK protein stimulates soft agar colony formation in vitro and tumor growth of SW-13 cells in athymic nude mice, ... [more]

J. Biol. Chem. Sep. 27, 2002; 277(39);35990-8 [Pubmed: 12122009]

Throughput

  • Low Throughput

Curated By

  • BioGRID