BET1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
COG2
Gene Ontology Biological Process
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Interaction of the conserved oligomeric Golgi complex with t-SNARE Syntaxin5a/Sed5 enhances intra-Golgi SNARE complex stability.
Tethering factors mediate initial interaction of transport vesicles with target membranes. Soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptors (SNAREs) enable consequent docking and membrane fusion. We demonstrate that the vesicle tether conserved oligomeric Golgi (COG) complex colocalizes and coimmunoprecipitates with intra-Golgi SNARE molecules. In yeast cells, the COG complex preferentially interacts with the SNARE complexes containing yeast Golgi target (t)-SNARE ... [more]
Throughput
- Low Throughput
Additional Notes
- GST pull-down of purified recombinant proteins. GST-BET1 was pre-incubated with His-Sed5,His-Bos1 and His-Sec22, then bound to glutathione-Sepharose beads, then incubated with the GOG complex.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| COG2 BET1 | Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene. | Low | - | BioGRID | 154088 | |
| BET1 COG2 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1652 | BioGRID | 1937246 |
Curated By
- BioGRID