BAIT
CBL
C-CBL, CBL2, FRA11B, NSLL, RNF55
Cbl proto-oncogene, E3 ubiquitin protein ligase
GO Process (10)
GO Function (5)
GO Component (4)
Gene Ontology Biological Process
- cell surface receptor signaling pathway [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- negative regulation of apoptotic process [IMP]
- negative regulation of epidermal growth factor receptor signaling pathway [TAS]
- positive regulation of phosphatidylinositol 3-kinase signaling [IMP]
- positive regulation of receptor-mediated endocytosis [TAS]
- protein ubiquitination [TAS]
- regulation of transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
UBE2E2
UBCH8
ubiquitin-conjugating enzyme E2E 2
GO Process (6)
GO Function (4)
GO Component (0)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Cbl interacts with multiple E2s in vitro and in cells.
Many receptor tyrosine kinases (RTKs, such as EGFR, MET) are negatively regulated by ubiquitination and degradation mediated by Cbl proteins, a family of RING finger (RF) ubiquitin ligases (E3s). Loss of Cbl protein function is associated with malignant transformation driven by increased RTK activity. RF E3s, such as the Cbl proteins, interact with a ubiquitin-conjugating enzyme (E2) to confer specificity ... [more]
PLoS ONE May. 24, 2019; 14(5);e0216967 [Pubmed: 31120930]
Throughput
- Low Throughput
Additional Notes
- The activated Cbl (Y371E) mutant was used in the yeast two-hybrid screen to capture transient or low affinity interactions.
Curated By
- BioGRID