BAIT

HYPK

C15orf63, HSPC136
huntingtin interacting protein K
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

HSPA9

CRP40, CSA, GRP-75, GRP75, HEL-S-124m, HSPA9B, MOT, MOT2, MTHSP75, PBP74
heat shock 70kDa protein 9 (mortalin)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Chaperone-like protein HYPK and its interacting partners augment autophagy.

Choudhury KR, Bucha S, Baksi S, Mukhopadhyay D, Bhattacharyya NP

To decipher the function(s) of HYPK, a huntingtin (HTT)-interacting protein with chaperone-like activity, we had previously identified 36 novel interacting partners of HYPK. Another 13 proteins were known earlier to be associated with HYPK. On the basis of analysis of the interacting partners of HYPK, it has been shown that HYPK may participate in diverse cellular functions relevant to Huntington's ... [more]

Eur. J. Cell Biol. Apr. 14, 2016; 95(6-7);182-94 [Pubmed: 27067261]

Throughput

  • Low Throughput

Curated By

  • BioGRID