An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A Human Tyrosine Phosphatase Interactome Mapped by Proteomic Profiling.

Kumar P, Munnangi P, Chowdary KR, Shah VJ, Shinde SR, Kolli NR, Halehalli RR, Nagarajaram HA, Maddika S

Tyrosine phosphatases play a critical role in many cellular processes and pathogenesis, yet comprehensive analysis of their functional interacting proteins in the cell is limited. By utilizing a proteomic approach, here we present an interaction network of 81 human tyrosine phosphatases built on 1884 high-confidence interactions of which 85% are unreported. Our analysis has linked several phosphatases with new cellular ... [more]

J. Proteome Res. Dec. 04, 2016; 16(8);2789-2801 [Pubmed: 28675297]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TENC1 CRKL	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Grossmann A (2015)	High	-	BioGRID	-
CRKL TENC1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Wang J (2011)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

TENC1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

CRKL