Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A Human Tyrosine Phosphatase Interactome Mapped by Proteomic Profiling.

Kumar P, Munnangi P, Chowdary KR, Shah VJ, Shinde SR, Kolli NR, Halehalli RR, Nagarajaram HA, Maddika S

Tyrosine phosphatases play a critical role in many cellular processes and pathogenesis, yet comprehensive analysis of their functional interacting proteins in the cell is limited. By utilizing a proteomic approach, here we present an interaction network of 81 human tyrosine phosphatases built on 1884 high-confidence interactions of which 85% are unreported. Our analysis has linked several phosphatases with new cellular ... [more]

J. Proteome Res. Dec. 04, 2016; 16(8);2789-2801 [Pubmed: 28675297]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TENC1 SNTA1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
2296594

Curated By

  • BioGRID