TH
Gene Ontology Biological Process
- catecholamine biosynthetic process [IDA]
- cellular response to alkaloid [IEP]
- cellular response to drug [IEP]
- cellular response to glucose stimulus [IEP]
- cellular response to growth factor stimulus [IEP]
- cellular response to manganese ion [IEP]
- cellular response to nicotine [IEP]
- cerebral cortex development [IEP]
- circadian sleep/wake cycle [IEP]
- cognition [IEP]
- dopamine biosynthetic process [ISO]
- dopamine biosynthetic process from tyrosine [IDA, IMP, ISO]
- eating behavior [ISO]
- embryonic camera-type eye morphogenesis [ISO]
- epinephrine biosynthetic process [ISO]
- eye photoreceptor cell development [ISO]
- fatty acid metabolic process [IEP]
- glycoside metabolic process [IEP]
- heart development [IDA, ISO]
- isoquinoline alkaloid metabolic process [IEP]
- learning [ISO]
- locomotory behavior [ISO]
- mating behavior [ISO]
- memory [ISO]
- multicellular organismal aging [IEP]
- norepinephrine biosynthetic process [ISO]
- organ morphogenesis [ISO]
- phthalate metabolic process [IEP]
- phytoalexin metabolic process [IEP]
- protein homotetramerization [TAS]
- regulation of heart contraction [ISO]
- response to activity [IEP]
- response to amphetamine [IEP]
- response to corticosterone [IEP]
- response to drug [IEP]
- response to electrical stimulus [IEP]
- response to estradiol [IEP]
- response to ethanol [IEP, ISO]
- response to ether [IEP]
- response to growth factor [IEP]
- response to herbicide [IEP]
- response to hypoxia [IDA, IEP, ISO]
- response to immobilization stress [IEP]
- response to insecticide [IEP]
- response to isolation stress [IEP]
- response to light stimulus [IEP]
- response to lipopolysaccharide [IEP]
- response to metal ion [IEP]
- response to nicotine [IEP]
- response to nutrient levels [IEP]
- response to organic cyclic compound [IEP]
- response to peptide hormone [IEP]
- response to pyrethroid [IEP]
- response to salt stress [IEP]
- response to steroid hormone [IEP]
- response to water deprivation [IEP]
- response to zinc ion [IEP]
- sensory perception of sound [IEP]
- social behavior [IEP]
- sphingolipid metabolic process [IEP]
- synaptic transmission, dopaminergic [ISO]
- synaptic vesicle amine transport [IDA]
- terpene metabolic process [IEP]
- visual perception [ISO]
Gene Ontology Molecular Function- amino acid binding [IDA, IPI]
- dopamine binding [IPI]
- enzyme binding [ISO]
- ferric iron binding [IDA]
- ferrous iron binding [IDA]
- monooxygenase activity [IDA]
- oxygen binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- tetrahydrobiopterin binding [IDA]
- tyrosine 3-monooxygenase activity [IDA, ISO]
- amino acid binding [IDA, IPI]
- dopamine binding [IPI]
- enzyme binding [ISO]
- ferric iron binding [IDA]
- ferrous iron binding [IDA]
- monooxygenase activity [IDA]
- oxygen binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- tetrahydrobiopterin binding [IDA]
- tyrosine 3-monooxygenase activity [IDA, ISO]
Gene Ontology Cellular Component
- axon [IDA, ISO]
- cytoplasm [IDA, ISO]
- cytoplasmic side of plasma membrane [ISO]
- cytoplasmic vesicle [ISO]
- cytoplasmic vesicle membrane [IDA]
- cytosol [IDA]
- dendrite [IDA]
- melanosome membrane [ISO]
- mitochondrion [IDA]
- neuron projection [IDA, ISO]
- neuronal cell body [IDA]
- nucleus [ISO]
- perikaryon [IDA, ISO]
- smooth endoplasmic reticulum [ISO]
- synaptic vesicle [IDA]
- terminal bouton [IDA]
SLC18A2
Gene Ontology Biological Process
- aging [IDA]
- cellular response to ammonium ion [IEP]
- cellular response to drug [IDA]
- death [ISO]
- endocytic recycling [IEP]
- glucose homeostasis [IDA]
- insulin secretion [IDA]
- locomotory behavior [ISO]
- monoamine transport [IDA]
- negative regulation of neurotransmitter transport [IMP]
- neurotransmitter transport [IDA]
- post-embryonic development [ISO]
- response to amphetamine [IEP, ISO]
- response to cocaine [IEP]
- response to corticosterone [IEP]
- response to drug [IEP]
- response to herbicide [IEP]
- response to metal ion [IEP]
- response to starvation [IEP]
- response to toxic substance [ISO]
- response to zinc ion [IEP]
- serotonin transport [IDA]
- synaptic vesicle amine transport [IMP, TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
A biochemical and functional protein complex involving dopamine synthesis and transport into synaptic vesicles.
Synaptic transmission depends on neurotransmitter pools stored within vesicles that undergo regulated exocytosis. In the brain, the vesicular monoamine transporter-2 (VMAT(2)) is responsible for the loading of dopamine (DA) and other monoamines into synaptic vesicles. Prior to storage within vesicles, DA synthesis occurs at the synaptic terminal in a two-step enzymatic process. First, the rate-limiting enzyme tyrosine hydroxylase (TH) converts ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SLC18A2 TH | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID