An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interactome analyses revealed that the U1 snRNP machinery overlaps extensively with the RNAP II machinery and contains multiple ALS/SMA-causative proteins.

Chi B, O'Connell JD, Yamazaki T, Gangopadhyay J, Gygi SP, Reed R

Mutations in multiple RNA/DNA binding proteins cause Amyotrophic Lateral Sclerosis (ALS). Included among these are the three members of the FET family (FUS, EWSR1 and TAF15) and the structurally similar MATR3. Here, we characterized the interactomes of these four proteins, revealing that they largely have unique interactors, but share in common an association with U1 snRNP. The latter observation led ... [more]

Sci Rep Dec. 08, 2017; 8(1);8755 [Pubmed: 29884807]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SNRPC SNRPC	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Horlbeck MA (2018)	High	-4.6931	BioGRID	2458832

Curated By

BioGRID

Interaction Summary

SNRPC

Gene Ontology Biological Process

Gene Ontology Molecular Function

U1 snRNA binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]protein homodimerization activity [IPI]single-stranded RNA binding [IDA]zinc ion binding [IDA]

Gene Ontology Cellular Component