An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

Proteomic analysis of the U1 snRNP of Schizosaccharomyces pombe reveals three essential organism-specific proteins.

Newo AN, Luetzelberger M, Bottner CA, Wehland J, Wissing J, Jaensch L, Kaeufer NF

Characterization of spliceosomal complexes in the fission yeast Schizosaccharomyces pombe revealed particles sedimenting in the range of 30-60S, exclusively containing U1 snRNA. Here, we report the tandem affinity purification (TAP) of U1-specific protein complexes. The components of the complexes were identified using (LC-MS/MS) mass spectrometry. The fission yeast U1 snRNP contains 16 proteins, including the 7 Sm snRNP core proteins. ... [more]

Nucleic Acids Res. Feb. 01, 2007; 35(5);1391-401 [Pubmed: 17264129]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

USP101

Gene Ontology Biological Process

Gene Ontology Molecular Function

U1 snRNA binding [ISO]mRNA binding [ISO]

Gene Ontology Cellular Component

USP105