Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Synaptic localization of ?5 GABA (A) receptors via gephyrin interaction regulates dendritic outgrowth and spine maturation.

Brady ML, Jacob TC

GABAA receptor subunit composition is a critical determinant of receptor localization and physiology, with synaptic receptors generating phasic inhibition and extrasynaptic receptors producing tonic inhibition. Extrasynaptically localized ?5 GABAA receptors are largely responsible for tonic inhibition in hippocampal neurons. However, we show here that inhibitory synapses also contain a constant level of ?5 GABAA receptors throughout neuronal development, as measured ... [more]

Dev Neurobiol Nov. 01, 2015; 75(11);1241-51 [Pubmed: 25663431]

Throughput

  • Low Throughput

Curated By

  • BioGRID