BAIT
SUMO1
DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1, OK/SW-cl.43
small ubiquitin-like modifier 1
GO Process (14)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
- DNA repair [TAS]
- cellular protein metabolic process [TAS]
- cytokine-mediated signaling pathway [TAS]
- interferon-gamma-mediated signaling pathway [TAS]
- negative regulation of DNA binding [IMP]
- negative regulation of sequence-specific DNA binding transcription factor activity [IMP]
- negative regulation of transcription, DNA-templated [IDA, IMP]
- palate development [ISS]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein complex assembly [IDA]
- post-translational protein modification [TAS]
- protein sumoylation [IDA, TAS]
- regulation of interferon-gamma-mediated signaling pathway [TAS]
- regulation of protein localization [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
TDG
E130317C12Rik, JZA-3, Jza1
thymine DNA glycosylase
GO Process (9)
GO Function (12)
GO Component (4)
Gene Ontology Biological Process
- DNA demethylation [IDA, IMP]
- base-excision repair [IDA, ISO]
- embryo development [IMP]
- mismatch repair [ISO]
- negative regulation of chromatin binding [IDA]
- negative regulation of protein binding [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IGI, IMP, ISO]
- regulation of gene expression, epigenetic [IMP]
- transcription from RNA polymerase II promoter [IGI]
Gene Ontology Molecular Function- DNA N-glycosylase activity [IDA, ISO]
- RNA polymerase II transcription cofactor activity [IGI]
- SUMO binding [IPI]
- double-stranded DNA binding [ISO]
- mismatched DNA binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein homodimerization activity [ISO]
- protein kinase C binding [IPI]
- pyrimidine-specific mismatch base pair DNA N-glycosylase activity [ISO]
- structure-specific DNA binding [IDA]
- transcription factor binding [ISO]
- DNA N-glycosylase activity [IDA, ISO]
- RNA polymerase II transcription cofactor activity [IGI]
- SUMO binding [IPI]
- double-stranded DNA binding [ISO]
- mismatched DNA binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein homodimerization activity [ISO]
- protein kinase C binding [IPI]
- pyrimidine-specific mismatch base pair DNA N-glycosylase activity [ISO]
- structure-specific DNA binding [IDA]
- transcription factor binding [ISO]
Gene Ontology Cellular Component
- PML body [IDA]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
- plasma membrane [ISO]
Mus musculus
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Noncovalent SUMO-1 binding activity of thymine DNA glycosylase (TDG) is required for its SUMO-1 modification and colocalization with the promyelocytic leukemia protein.
SUMO-1 is a member of a family of ubiquitin-like molecules that are post-translationally conjugated to various cellular proteins in a process that is mechanistically similar to ubiquitylation. To identify molecules that bind noncovalently to SUMO-1, we performed yeast two-hybrid screening with a SUMO-1 mutant that cannot be conjugated to target proteins as the bait. This screening resulted in the isolation ... [more]
J. Biol. Chem. Feb. 18, 2005; 280(7);5611-21 [Pubmed: 15569683]
Throughput
- Low Throughput
Additional Notes
- #LPPI
- Identified proteins that bind SUMO-1 noncovalently by using a SUMO-1 mutant that cannot be conjugated to proteins.
- Likely protein-protein interaction
Curated By
- BioGRID