UBE2I
Gene Ontology Biological Process
- cellular protein modification process [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IMP, ISO]
- negative regulation of transcription, DNA-templated [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- positive regulation of intracellular steroid hormone receptor signaling pathway [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO]
- protein sumoylation [IBA, ISO]
- protein ubiquitination [IBA, ISO]
- regulation of receptor activity [ISO]
Gene Ontology Molecular Function- HLH domain binding [IPI]
- RING-like zinc finger domain binding [ISO]
- SUMO transferase activity [IBA, ISO]
- bHLH transcription factor binding [ISO]
- enzyme binding [ISO]
- poly(A) RNA binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- transcription factor binding [ISO]
- ubiquitin-protein transferase activity [ISO]
- HLH domain binding [IPI]
- RING-like zinc finger domain binding [ISO]
- SUMO transferase activity [IBA, ISO]
- bHLH transcription factor binding [ISO]
- enzyme binding [ISO]
- poly(A) RNA binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- transcription factor binding [ISO]
- ubiquitin-protein transferase activity [ISO]
TDG
Gene Ontology Biological Process
- DNA demethylation [IDA, IMP]
- base-excision repair [IDA, ISO]
- embryo development [IMP]
- mismatch repair [ISO]
- negative regulation of chromatin binding [IDA]
- negative regulation of protein binding [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IGI, IMP, ISO]
- regulation of gene expression, epigenetic [IMP]
- transcription from RNA polymerase II promoter [IGI]
Gene Ontology Molecular Function- DNA N-glycosylase activity [IDA, ISO]
- RNA polymerase II transcription cofactor activity [IGI]
- SUMO binding [IPI]
- double-stranded DNA binding [ISO]
- mismatched DNA binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein homodimerization activity [ISO]
- protein kinase C binding [IPI]
- pyrimidine-specific mismatch base pair DNA N-glycosylase activity [ISO]
- structure-specific DNA binding [IDA]
- transcription factor binding [ISO]
- DNA N-glycosylase activity [IDA, ISO]
- RNA polymerase II transcription cofactor activity [IGI]
- SUMO binding [IPI]
- double-stranded DNA binding [ISO]
- mismatched DNA binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein homodimerization activity [ISO]
- protein kinase C binding [IPI]
- pyrimidine-specific mismatch base pair DNA N-glycosylase activity [ISO]
- structure-specific DNA binding [IDA]
- transcription factor binding [ISO]
Gene Ontology Cellular Component
- PML body [IDA]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
- plasma membrane [ISO]
Biochemical Activity (Sumoylation)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
Noncovalent SUMO-1 binding activity of thymine DNA glycosylase (TDG) is required for its SUMO-1 modification and colocalization with the promyelocytic leukemia protein.
SUMO-1 is a member of a family of ubiquitin-like molecules that are post-translationally conjugated to various cellular proteins in a process that is mechanistically similar to ubiquitylation. To identify molecules that bind noncovalently to SUMO-1, we performed yeast two-hybrid screening with a SUMO-1 mutant that cannot be conjugated to target proteins as the bait. This screening resulted in the isolation ... [more]
Throughput
- Low Throughput
Additional Notes
- The reaction contained E1 (Aos1-Uba2), E2 (Ubc9), and SUMO-1.
Curated By
- BioGRID