An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Revealing A-Raf functions through its interactome.

Zhang XY, Guo H, Han B, Zhang XM, Huang Y, Yang Y, Liu Y, Guo XX, Hao Q, An S, Xu TR

A-Raf is a member of the Raf kinase family. Unlike B-Raf and C-Raf, the functions of A-Raf remain obscure. To gain more insight into the biological functions of A-Raf, we investigated the A-Raf interactome using proteomics. We found 132 proteins that interact with A-Raf and confirmed the interaction of 12 of these proteins with A-Raf by western blotting. Our data ... [more]

Biochim Biophys Acta Proteins Proteom Dec. 01, 2017; 1866(8);849-856 [Pubmed: 29777862]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

ARAF

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein kinase activity [TAS]protein serine/threonine kinase activity [IDA]

EEF1A1

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTP binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase binding [IPI]translation elongation factor activity [TAS]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

Revealing A-Raf functions through its interactome.

Throughput

Curated By

protein binding [IPI]
protein kinase activity [TAS]
protein serine/threonine kinase activity [IDA]

GTP binding [TAS]
poly(A) RNA binding [IDA]
protein binding [IPI]
protein kinase binding [IPI]
translation elongation factor activity [TAS]