BAIT

OTUD4

DUBA6, HIN1, HSHIN1
OTU deubiquitinase 4
UPS Project
GO Process (1)
GO Function (2)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens
PREY

RYR2

9330127I20Rik, RYR-2
ryanodine receptor 2, cardiac
GO Process (40)
GO Function (16)
GO Component (21)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

New roles for the de-ubiquitylating enzyme OTUD4 in an RNA-protein network and RNA granules.

Das R, Schwintzer L, Vinopal S, Aguado Roca E, Sylvester M, Oprisoreanu AM, Schoch S, Bradke F, Broemer M

Mechanisms that regulate the formation of membrane-less cellular organelles, such as neuronal RNA granules and stress granules, have gained increasing attention over the past years. These granules consist of RNA and a plethora of RNA-binding proteins. Mutations in RNA-binding proteins have been found in neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). By performing pulldown experiments ... [more]

J. Cell. Sci. Jun. 17, 2019; 132(12); [Pubmed: 31138677]

Throughput

  • High Throughput

Curated By

  • BioGRID