Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

SIP/CacyBP promotes autophagy by regulating levels of BRUCE/Apollon, which stimulates LC3-I degradation.

Jiang TX, Zou JB, Zhu QQ, Liu CH, Wang GF, Du TT, Luo ZY, Guo F, Zhou LM, Liu JJ, Zhang W, Shu YS, Yu L, Li P, Ronai ZA, Matsuzawa SI, Goldberg AL, Qiu XB

BRUCE/Apollon is a membrane-associated inhibitor of apoptosis protein that is essential for viability and has ubiquitin-conjugating activity. On initiation of apoptosis, the ubiquitin ligase Nrdp1/RNF41 promotes proteasomal degradation of BRUCE. Here we demonstrate that BRUCE together with the proteasome activator PA28? causes proteasomal degradation of LC3-I and thus inhibits autophagy. LC3-I on the phagophore membrane is conjugated to phosphatidylethanolamine to ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 02, 2018; 116(27);13404-13413 [Pubmed: 31213539]

Throughput

  • Low Throughput

Curated By

  • BioGRID