TRIM32
Gene Ontology Biological Process
- fat cell differentiation [ISS]
- innate immune response [IDA, TAS]
- negative regulation of fibroblast proliferation [ISS]
- negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage [IDA]
- negative regulation of viral release from host cell [IDA]
- negative regulation of viral transcription [IDA]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- positive regulation of NF-kappaB transcription factor activity [IDA, ISS]
- positive regulation of cell cycle [IDA]
- positive regulation of cell growth [IDA]
- positive regulation of cell migration [IDA]
- positive regulation of cell motility [ISS]
- positive regulation of neurogenesis [ISS]
- positive regulation of neuron differentiation [ISS]
- positive regulation of protein catabolic process [ISS]
- positive regulation of proteolysis [IDA]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of type I interferon production [TAS]
- protein polyubiquitination [IDA]
- protein ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IMP]
- regulation of type I interferon production [TAS]
- response to UV [ISS]
- response to tumor necrosis factor [ISS]
Gene Ontology Molecular Function
ULK1
Gene Ontology Biological Process
- Ras protein signal transduction [IDA]
- autophagic vacuole assembly [IMP]
- axon extension [IMP]
- axonogenesis [IDA]
- cellular response to nutrient levels [IDA]
- cerebellar granule cell differentiation [IMP]
- negative regulation of collateral sprouting [IMP]
- neuron projection development [IMP, ISO]
- neuron projection regeneration [ISO]
- positive regulation of autophagy [IMP]
- positive regulation of macroautophagy [ISO]
- protein autophosphorylation [IDA, ISO]
- protein localization [ISO]
- radial glia guided migration of cerebellar granule cell [IMP]
- receptor internalization [IMP]
- regulation of autophagy [IMP, ISO]
- regulation of neurotrophin TRK receptor signaling pathway [IMP]
- response to starvation [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- ATG1/UKL1 signaling complex [IBA]
- ULK1-ATG13-FIP200 complex [IPI, ISO]
- autophagic vacuole [ISO]
- cytoplasm [IDA]
- cytoplasmic vesicle membrane [IDA]
- cytosol [IDA, ISO]
- extrinsic component of autophagic vacuole membrane [ISO]
- extrinsic component of omegasome membrane [ISO]
- extrinsic component of pre-autophagosomal structure membrane [ISO]
- membrane [IDA]
- neuron projection [IDA]
- neuronal cell body [IDA]
- pre-autophagosomal structure [IDA]
- pre-autophagosomal structure membrane [IDA, ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains.
Optimal autophagic activity is crucial to maintain muscle integrity, with either reduced or excessive levels leading to specific myopathies. LGMD2H is a muscle dystrophy caused by mutations in the ubiquitin ligase TRIM32, whose function in muscles remains not fully understood. Here, we show that TRIM32 is required for the induction of muscle autophagy in atrophic conditions using both in vitro ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID