Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The interactome of the prostate-specific protein Anoctamin 7.

Kaikkonen E, Takala A, Pursiheimo JP, Wahlstroem G, Schleutker J

Elevated Anoctamin 7 (ANO7) expression is associated with poor survival in prostate cancer patients.The aim was to discover proteins that interact with ANO7 to understand its functions and regulatory mechanisms.The proximity-dependent biotin identification (BioID) method was utilized. ANO7 fused to biotin ligase was transiently transfected into LNCaP cells, and the biotinylated proteins were collected and analysed by mass spectrometry. Four ... [more]

Cancer Biomark Mar. 06, 2020; (); [Pubmed: 32176628]

Throughput

  • High Throughput

Curated By

  • BioGRID