PREY

CASP3

CPP32, CPP32B, SCA-1
caspase 3, apoptosis-related cysteine peptidase
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A Multidimensional Characterization of E3 Ubiquitin Ligase and Substrate Interaction Network.

Chen D, Liu X, Xia T, Tekcham DS, Wang W, Chen H, Li T, Lu C, Ning Z, Liu X, Liu J, Qi H, He H, Piao HL

E3 ubiquitin ligases (E3s) play a critical role in molecular and cellular mechanisms. However, a large number of E3-substrate interactions (ESIs) remain unrevealed. Here, we integrated the increasing omics data with biological knowledge to characterize and identify ESIs. Multidimensional features were computed to obtain the association patterns of ESIs, and an ensemble prediction model was constructed to identify ESIs. Comparison ... [more]

iScience Jun. 28, 2019; 16();177-191 [Pubmed: 31181401]

Throughput

  • Low Throughput

Curated By

  • BioGRID