Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

LMBR1L regulates lymphopoiesis through Wnt/?-catenin signaling.

Choi JH, Zhong X, McAlpine W, Liao TC, Zhang D, Fang B, Russell J, Ludwig S, Nair-Gill E, Zhang Z, Wang KW, Misawa T, Zhan X, Choi M, Wang T, Li X, Tang M, Sun Q, Yu L, Murray AR, Moresco EMY, Beutler B

Precise control of Wnt signaling is necessary for immune system development. In this study, we detected severely impaired development of all lymphoid lineages in mice, resulting from an N-ethyl-N-nitrosourea-induced mutation in the limb region 1-like gene (Lmbr1l), which encodes a membrane-spanning protein with no previously described function in immunity. The interaction of LMBR1L with glycoprotein 78 (GP78) and ubiquitin-associated domain-containing ... [more]

Science Dec. 10, 2018; 364(6440); [Pubmed: 31073040]

Throughput

  • High Throughput

Additional Notes

  • hits are proteins whose spectral counts in an immunoprecipitation (IP) with the bait protein is four or more times higher than the count in a vector only IP
  • hits are proteins whose spectral counts in immunoprecipitation (IP) of the bait is four or more times higher than the count in a vector control IP

Curated By

  • BioGRID