BAIT
MFN2
CMT2A, CMT2A2, CPRP1, HSG, MARF, RP5-1077B9.3
mitofusin 2
GO Process (9)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
- blood coagulation [TAS]
- cell cycle arrest [ISS]
- mitochondrial fusion [IMP, ISS, NAS]
- mitochondrial membrane organization [IDA]
- mitochondrion localization [IDA]
- negative regulation of Ras protein signal transduction [IDA]
- negative regulation of smooth muscle cell proliferation [ISS]
- protein localization to pre-autophagosomal structure [IDA]
- protein targeting to mitochondrion [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
LRRK2
AURA17, DARDARIN, PARK8, RIPK7, ROCO2
leucine-rich repeat kinase 2
GO Process (61)
GO Function (21)
GO Component (23)
Gene Ontology Biological Process
- GTP catabolic process [IDA]
- MAPK cascade [IDA]
- activation of MAPK activity [IDA]
- activation of MAPKK activity [IDA]
- cellular protein localization [ISS]
- cellular response to dopamine [IMP]
- cellular response to manganese ion [IMP]
- cellular response to oxidative stress [IMP]
- determination of adult lifespan [IMP]
- endocytosis [IGI, IMP]
- exploration behavior [IMP]
- intracellular distribution of mitochondria [IMP]
- intracellular signal transduction [ISS]
- negative regulation of GTPase activity [IDA]
- negative regulation of hydrogen peroxide-induced cell death [IMP]
- negative regulation of late endosome to lysosome transport [TAS]
- negative regulation of peroxidase activity [IDA]
- negative regulation of protein binding [IMP]
- negative regulation of protein phosphorylation [ISS]
- negative regulation of protein processing [IDA]
- negative regulation of protein processing involved in protein targeting to mitochondrion [IC]
- negative regulation of protein targeting to mitochondrion [IDA]
- negative regulation of thioredoxin peroxidase activity by peptidyl-threonine phosphorylation [IDA]
- neuromuscular junction development [IMP]
- neuron death [IMP]
- neuron projection morphogenesis [IMP]
- olfactory bulb development [IMP]
- peptidyl-serine phosphorylation [IDA]
- peptidyl-threonine phosphorylation [IDA, IMP]
- positive regulation of GTPase activity [IDA]
- positive regulation of MAP kinase activity [IC]
- positive regulation of autophagy [IMP, ISS]
- positive regulation of dopamine receptor signaling pathway [IMP]
- positive regulation of programmed cell death [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISS]
- positive regulation of protein autoubiquitination [IDA]
- positive regulation of protein binding [IDA]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of protein ubiquitination [IDA]
- protein autophosphorylation [IDA, IMP]
- protein localization to mitochondrion [TAS]
- protein phosphorylation [IMP]
- reactive oxygen species metabolic process [IMP]
- regulation of branching morphogenesis of a nerve [IMP]
- regulation of dendritic spine morphogenesis [IMP, ISS]
- regulation of dopamine receptor signaling pathway [ISS]
- regulation of excitatory postsynaptic membrane potential [ISS]
- regulation of kidney size [ISS]
- regulation of locomotion [IMP]
- regulation of membrane potential [IMP]
- regulation of mitochondrial depolarization [IMP]
- regulation of mitochondrial fission [TAS]
- regulation of neuroblast proliferation [IMP]
- regulation of neuron death [IMP]
- regulation of neuron maturation [IMP]
- regulation of protein kinase A signaling [ISS]
- regulation of synaptic transmission, glutamatergic [ISS]
- regulation of synaptic vesicle exocytosis [IMP]
- regulation of synaptic vesicle transport [ISS, TAS]
- response to oxidative stress [IMP]
- tangential migration from the subventricular zone to the olfactory bulb [IMP]
Gene Ontology Molecular Function- GTP binding [IDA]
- GTP-dependent protein kinase activity [IDA, IMP]
- GTPase activator activity [IDA]
- GTPase activity [IDA]
- MAP kinase kinase activity [IDA]
- Rho GTPase binding [IPI]
- SNARE binding [IPI]
- actin binding [IPI]
- clathrin binding [IPI]
- glycoprotein binding [IPI]
- identical protein binding [IPI]
- ion channel binding [IPI]
- kinase activity [IDA]
- peroxidase inhibitor activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- protein kinase A binding [IPI]
- protein kinase activity [IDA]
- protein serine/threonine kinase activity [IDA]
- syntaxin-1 binding [IPI]
- tubulin binding [IDA]
- GTP binding [IDA]
- GTP-dependent protein kinase activity [IDA, IMP]
- GTPase activator activity [IDA]
- GTPase activity [IDA]
- MAP kinase kinase activity [IDA]
- Rho GTPase binding [IPI]
- SNARE binding [IPI]
- actin binding [IPI]
- clathrin binding [IPI]
- glycoprotein binding [IPI]
- identical protein binding [IPI]
- ion channel binding [IPI]
- kinase activity [IDA]
- peroxidase inhibitor activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- protein kinase A binding [IPI]
- protein kinase activity [IDA]
- protein serine/threonine kinase activity [IDA]
- syntaxin-1 binding [IPI]
- tubulin binding [IDA]
Gene Ontology Cellular Component
- Golgi apparatus [ISS]
- axon [IDA, ISS]
- cytoplasm [IDA]
- cytoplasmic side of mitochondrial outer membrane [IDA]
- cytoplasmic vesicle [ISS]
- cytosol [IDA]
- dendrite [IDA, ISS]
- dendrite cytoplasm [IDA]
- endoplasmic reticulum [ISS]
- endosome [ISS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- inclusion body [IMP]
- lysosome [ISS]
- mitochondrial inner membrane [ISS]
- mitochondrial matrix [ISS]
- mitochondrial membrane [IDA]
- mitochondrial outer membrane [ISS]
- mitochondrion [IDA]
- neuron projection [IDA]
- neuronal cell body [IDA]
- perikaryon [IDA, ISS]
- terminal bouton [TAS]
Homo sapiens
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Functional interaction of Parkinson's disease-associated LRRK2 with members of the dynamin GTPase superfamily.
Mutations in LRRK2 cause autosomal dominant Parkinson's disease (PD). LRRK2 encodes a multi-domain protein containing GTPase and kinase domains, and putative protein-protein interaction domains. Familial PD mutations alter the GTPase and kinase activity of LRRK2 in vitro. LRRK2 is suggested to regulate a number of cellular pathways although the underlying mechanisms are poorly understood. To explore such mechanisms, it has ... [more]
Hum. Mol. Genet. Apr. 15, 2014; 23(8);2055-77 [Pubmed: 24282027]
Throughput
- Low Throughput
Curated By
- BioGRID