BAIT
NFX1
NFX2, TEG-42, Tex42
nuclear transcription factor, X-box binding 1
GO Process (3)
GO Function (4)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- poly(A) RNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- poly(A) RNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [TAS]
Gene Ontology Cellular Component
Homo sapiens
PREY
PHB
HEL-215, HEL-S-54e, PHB1
prohibitin
GO Process (15)
GO Function (4)
GO Component (8)
Gene Ontology Biological Process
- cellular response to interleukin-6 [IDA]
- histone deacetylation [IDA]
- negative regulation of androgen receptor signaling pathway [IDA, IMP]
- negative regulation of cell growth [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of glucocorticoid receptor signaling pathway [IDA]
- negative regulation of transcription by competitive promoter binding [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA, IMP]
- osteoblast differentiation [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- progesterone receptor signaling pathway [IDA]
- regulation of apoptotic process [TAS]
- regulation of transcription, DNA-templated [IDA]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Identification of a USP9X Substrate NFX1-123 by SILAC-Based Quantitative Proteomics.
The deubiquitinase USP9X is involved in multiple diseases including neurodegeneration, epilepsy, and various types of tumors by targeting different substrates. In the present study, we aimed to explore the potential substrates of USP9X and performed SILAC-based quantitative proteomics to compare these substrates in USP9X-knockdown and wild-type HeLa cells. We consequently carried out Flag-NFX1-123 tag affinity-based mass spectrometry and confirmed that ... [more]
J. Proteome Res. Dec. 07, 2018; 18(6);2654-2665 [Pubmed: 31059266]
Throughput
- High Throughput
Curated By
- BioGRID