PREY

PSMC3

TBP1
proteasome (prosome, macropain) 26S subunit, ATPase, 3
GO Process (24)
GO Function (4)
GO Component (7)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification of a USP9X Substrate NFX1-123 by SILAC-Based Quantitative Proteomics.

Chen X, Lu D, Gao J, Zhu H, Zhou Y, Gao D, Zhou H

The deubiquitinase USP9X is involved in multiple diseases including neurodegeneration, epilepsy, and various types of tumors by targeting different substrates. In the present study, we aimed to explore the potential substrates of USP9X and performed SILAC-based quantitative proteomics to compare these substrates in USP9X-knockdown and wild-type HeLa cells. We consequently carried out Flag-NFX1-123 tag affinity-based mass spectrometry and confirmed that ... [more]

J. Proteome Res. Dec. 07, 2018; 18(6);2654-2665 [Pubmed: 31059266]

Throughput

  • High Throughput

Curated By

  • BioGRID