BAIT

RAD52

RP11-359B12.2
RAD52 homolog (S. cerevisiae)
GO Process (5)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

SHFM1

DSS1, ECD, SEM1, SHFD1, SHSF1, Shfdg1
split hand/foot malformation (ectrodactyly) type 1
Fanconi Anemia Project
UPS Project
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

DSS1 interacts with and stimulates RAD52 to promote the repair of DSBs.

Stefanovie B, Hengel SR, Mlcouskova J, Prochazkova J, Spirek M, Nikulenkov F, Nemecek D, Koch BG, Bain FE, Yu L, Spies M, Krejci L

The proper repair of deleterious DNA lesions such as double strand breaks prevents genomic instability and carcinogenesis. In yeast, the Rad52 protein mediates DSB repair via homologous recombination. In mammalian cells, despite the presence of the RAD52 protein, the tumour suppressor protein BRCA2 acts as the predominant mediator during homologous recombination. For decades, it has been believed that the RAD52 ... [more]

Nucleic Acids Res. Dec. 24, 2019; 48(2);694-708 [Pubmed: 31799622]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: chromosome/plasmid maintenance (APO:0000143)

Additional Notes

  • Figure 1C
  • break induced repair

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SHFM1 RAD52
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID