BAIT

WIPF3

CR16
WAS/WASL interacting protein family, member 3
GO Process (2)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

ITSN2

PRO2015, SH3D1B, SH3P18, SWA, SWAP
intersectin 2
GO Process (1)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Mammalian verprolin CR16 acts as a modulator of ITSN scaffold proteins association with actin.

Kropyvko S, Gryaznova T, Morderer D, Rynditch A

Actin cytoskeleton rearrangements are required for normal cell functioning, and their deregulation leads to various pathologies. Members of two mammalian protein families - ITSNs (ITSN1 and ITSN2) and verprolins (WIP, CR16 and WIRE) are involved in Cdc42/N-WASP/Arp2/3 signaling pathway-mediated remodeling of the actin cytoskeleton. Recently we demonstrated that ITSNs interact with the actin-regulating protein WIP. Here, we show that other ... [more]

Biochem. Biophys. Res. Commun. Dec. 18, 2016; 484(4);813-819 [Pubmed: 28161632]

Throughput

  • Low Throughput

Curated By

  • BioGRID