BAIT

GLN3

nitrogen-responsive transcriptional regulator GLN3, L000000710, YER040W
Transcriptional activator of genes regulated by nitrogen catabolite repression; localization and activity regulated by quality of nitrogen source and Ure2p
Saccharomyces cerevisiae (S288c)
PREY

ILV2

SMR1, THI1, acetolactate synthase catalytic subunit, L000002296, L000002515, L000000858, YMR108W
Acetolactate synthase; catalyses the first common step in isoleucine and valine biosynthesis and is the target of several classes of inhibitors, localizes to the mitochondria; expression of the gene is under general amino acid control
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Critical Roles of the Pentose Phosphate Pathway and GLN3 in Isobutanol-Specific Tolerance in Yeast.

Kuroda K, Hammer SK, Watanabe Y, Montano Lopez J, Fink GR, Stephanopoulos G, Ueda M, Avalos JL

Branched-chain alcohols are attractive advanced biofuels; however, their cellular toxicity is an obstacle to engineering microbes to produce them at high titers. We performed genome-wide screens on the Saccharomyces cerevisiae gene deletion library to identify cell systems involved in isobutanol-specific tolerance. Deletion of pentose phosphate pathway genes GND1 or ZWF1 causes hypersensitivity to isobutanol but not to ethanol. By contrast, ... [more]

Cell Syst Dec. 18, 2019; 9(6);534-547.e5 [Pubmed: 31734159]

Throughput

  • Low Throughput

Ontology Terms

  • chemical compound accumulation (APO:0000095)

Additional Notes

  • Co-overexpression of ILV2, ILV3, and ILV5 in gln3 deletion mutant increases isobutanol production
  • Figure 6
  • genetic complex

Curated By

  • BioGRID