BAIT

NSP2AB

nsp2, sars1ab, orf1ab, SARS-CoV nsp2ab, SARS-CoV nsp2, SARS-CoV sars1ab, orf1ab-nsp2, orf1ab-nsp2ab, pp1ab, p65 homolog, R1AB_CVHSA, PRO_0000037310, SARS-CoV-1ab-nsp2ab
Non-structural protein 2; nsp2; p65 homolog
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus
PREY

NSP8AB

nsp8, sars1ab, orf1ab, SARS-CoV nsp8ab, SARS-CoV nsp8, SARS-CoV sars1ab, orf1ab-nsp8, orf1ab-nsp8ab, pp1ab, R1AB_CVHSA, PRO_0000037315, SARS-CoV-1ab-nsp8ab
Non-structural protein 8; nsp8
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Analysis of intraviral protein-protein interactions of the SARS coronavirus ORFeome.

von Brunn A, Teepe C, Simpson JC, Pepperkok R, Friedel CC, Zimmer R, Roberts R, Baric R, Haas J

The severe acute respiratory syndrome coronavirus (SARS-CoV) genome is predicted to encode 14 functional open reading frames, leading to the expression of up to 30 structural and non-structural protein products. The functions of a large number of viral ORFs are poorly understood or unknown. In order to gain more insight into functions and modes of action and interaction of the ... [more]

PLoS ONE May. 23, 2007; 2(5);e459 [Pubmed: 17520018]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NSP8AB NSP2AB
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-
NSP2AB NSP8AB
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID