BAIT
PPP1R9B
Neb2
protein phosphatase 1, regulatory subunit 9B
GO Process (9)
GO Function (1)
GO Component (8)
Gene Ontology Biological Process
- actin filament organization [ISO]
- calcium-mediated signaling [ISO]
- cell migration [ISO, ISS]
- cellular response to morphine [ISO, ISS]
- dendrite development [ISO]
- filopodium assembly [ISO, ISS]
- negative regulation of cell growth [ISO]
- regulation of opioid receptor signaling pathway [ISO, ISS]
- regulation of protein phosphorylation [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
CAMK2B
Ck2b
calcium/calmodulin-dependent protein kinase II beta
GO Process (17)
GO Function (5)
GO Component (6)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [ISO]
- activation of meiosis involved in egg activation [ISO]
- calcium ion transport [ISO]
- cell projection morphogenesis [IMP]
- inhibitory G-protein coupled receptor phosphorylation [ISO]
- long-term synaptic potentiation [IMP]
- neuromuscular process controlling balance [ISO]
- peptidyl-serine phosphorylation [ISO]
- positive regulation of apoptotic signaling pathway [ISO]
- positive regulation of dendritic spine morphogenesis [IMP]
- positive regulation of neuron projection development [IMP]
- positive regulation of phospholipase A2 activity [IDA]
- positive regulation of synapse maturation [IMP]
- protein autophosphorylation [ISO, ISS]
- regulation of long-term neuronal synaptic plasticity [ISO]
- regulation of synaptic transmission, cholinergic [ISO]
- response to cadmium ion [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Identification and validation of novel spinophilin-associated proteins in rodent striatum using an enhanced ex vivo shotgun proteomics approach.
Spinophilin regulates excitatory postsynaptic function and morphology during development by virtue of its interactions with filamentous actin, protein phosphatase 1, and a plethora of additional signaling proteins. To provide insight into the roles of spinophilin in mature brain, we characterized the spinophilin interactome in subcellular fractions solubilized from adult rodent striatum by using a shotgun proteomics approach to identify proteins ... [more]
Mol. Cell Proteomics Jun. 01, 2010; 9(6);1243-59 [Pubmed: 20124353]
Throughput
- Low Throughput
Curated By
- BioGRID