Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

?4 Coordinates Small Intestinal Epithelium Homeostasis by Regulating Stability of HuR.

Chung HK, Wang SR, Xiao L, Rathor N, Turner DJ, Yang P, Gorospe M, Rao JN, Wang JY

The mammalian intestinal epithelium is a rapidly self-renewing tissue in the body, and its homeostasis depends on a dynamic balance among proliferation, migration, apoptosis, and differentiation of intestinal epithelial cells (IECs). The protein phosphatase 2A (PP2A)-associated protein ?4 controls the activity and specificity of serine/threonine phosphatases and is thus implicated in many cellular processes. Here, using a genetic approach, we ... [more]

Mol. Cell. Biol. Dec. 01, 2017; 38(11); [Pubmed: 29555726]

Throughput

  • Low Throughput

Curated By

  • BioGRID