An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

LCP1 preferentially binds clasped ?M?2 integrin and attenuates leukocyte adhesion under flow.

Tseng HY, Samarelli AV, Kammerer P, Scholze S, Ziegler T, Immler R, Zent R, Sperandio M, Sanders CR, Faessler R, Boettcher RT

Integrins are ?/? heterodimers that interconvert between inactive and active states. In the active state the ?/? cytoplasmic domains recruit integrin-activating proteins and separate the transmembrane and cytoplasmic (TMcyto) domains (unclasped TMcyto). Conversely, in the inactive state the ?/? TMcyto domains bind integrin-inactivating proteins, resulting in the association of the TMcyto domains (clasped TMcyto). Here, we report the isolation of ... [more]

J. Cell. Sci. Dec. 21, 2017; 131(22); [Pubmed: 30333137]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

ITGB2

Gene Ontology Biological Process

Gene Ontology Molecular Function

ICAM-3 receptor activity [ISO]cell adhesion molecule binding [ISO]glycoprotein binding [ISO]protein binding [IPI]protein complex binding [ISO]protein heterodimerization activity [ISO]protein kinase binding [ISO]

Gene Ontology Cellular Component

FLNA