DDX3X
Gene Ontology Biological Process
- ATP catabolic process [IDA, TAS]
- DNA duplex unwinding [IDA]
- RNA secondary structure unwinding [IDA]
- cellular response to arsenic-containing substance [IDA]
- cellular response to osmotic stress [IDA]
- chromosome segregation [IMP]
- extrinsic apoptotic signaling pathway via death domain receptors [IMP]
- innate immune response [IMP]
- intracellular signal transduction [IDA]
- intrinsic apoptotic signaling pathway [IMP]
- mature ribosome assembly [IMP]
- negative regulation of apoptotic process [IMP]
- negative regulation of cell growth [IDA]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of intrinsic apoptotic signaling pathway [IMP]
- negative regulation of protein complex assembly [IDA]
- negative regulation of translation [IMP]
- positive regulation of G1/S transition of mitotic cell cycle [IMP]
- positive regulation of apoptotic process [IMP]
- positive regulation of cell growth [IMP]
- positive regulation of chemokine (C-C motif) ligand 5 production [TAS]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- positive regulation of interferon-beta production [TAS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of translation [IDA]
- positive regulation of translational initiation [IMP]
- positive regulation of viral genome replication [IMP]
- response to virus [IDA]
- stress granule assembly [IDA]
Gene Ontology Molecular Function- ATP-dependent DNA helicase activity [IDA]
- ATP-dependent RNA helicase activity [IDA]
- ATPase activity [IDA]
- DNA binding [IDA]
- RNA binding [IDA]
- RNA stem-loop binding [IDA]
- eukaryotic initiation factor 4E binding [IDA]
- mRNA 5'-UTR binding [IDA]
- poly(A) RNA binding [IDA]
- poly(A) binding [IDA]
- protein binding [IPI]
- ribosomal small subunit binding [IDA]
- transcription factor binding [IDA]
- translation initiation factor binding [IDA]
- ATP-dependent DNA helicase activity [IDA]
- ATP-dependent RNA helicase activity [IDA]
- ATPase activity [IDA]
- DNA binding [IDA]
- RNA binding [IDA]
- RNA stem-loop binding [IDA]
- eukaryotic initiation factor 4E binding [IDA]
- mRNA 5'-UTR binding [IDA]
- poly(A) RNA binding [IDA]
- poly(A) binding [IDA]
- protein binding [IPI]
- ribosomal small subunit binding [IDA]
- transcription factor binding [IDA]
- translation initiation factor binding [IDA]
Gene Ontology Cellular Component
HNF4A
Gene Ontology Biological Process
- blood coagulation [IDA]
- endocrine pancreas development [TAS]
- gene expression [TAS]
- glucose homeostasis [ISS]
- lipid homeostasis [IMP]
- negative regulation of cell growth [IMP]
- negative regulation of cell proliferation [IMP]
- ornithine metabolic process [IMP]
- phospholipid homeostasis [ISS]
- positive regulation of cholesterol homeostasis [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of growth hormone receptor signaling pathway [NAS]
- regulation of insulin secretion [ISS]
- regulation of lipid metabolic process [IDA]
- regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- response to glucose [ISS]
- transcription initiation from RNA polymerase II promoter [TAS]
- triglyceride homeostasis [ISS]
- xenobiotic metabolic process [IMP]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [ISS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISS]
- fatty acid binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- receptor binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
- DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [ISS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISS]
- fatty acid binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- receptor binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
RNA helicase DDX3 maintains lipid homeostasis through upregulation of the microsomal triglyceride transfer protein by interacting with HNF4 and SHP.
Multifunctional RNA helicase DDX3 participates in HCV infection, one of the major causes of hepatic steatosis. Here, we investigated the role of DDX3 in hepatic lipid metabolism. We found that HCV infection severely reduced DDX3 expression. Analysis of intracellular triglyceride and secreted ApoB indicated that lipid accumulations were increased while ApoB secretion were decreased in DDX3 knockdown HuH7 and HepG2 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DDX3X HNF4A | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | 2661836 | |
| HNF4A DDX3X | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | 2661841 |
Curated By
- BioGRID