Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

BraInMap Elucidates the Macromolecular Connectivity Landscape of Mammalian Brain.

Pourhaghighi R, Ash PEA, Phanse S, Goebels F, Hu LZM, Chen S, Zhang Y, Wierbowski SD, Boudeau S, Moutaoufik MT, Malty RH, Malolepsza E, Tsafou K, Nathan A, Cromar G, Guo H, Abdullatif AA, Apicco DJ, Becker LA, Gitler AD, Pulst SM, Youssef A, Hekman R, Havugimana PC, White CA, Blum BC, Ratti A, Bryant CD, Parkinson J, Lage K, Babu M, Yu H, Bader GD, Wolozin B, Emili A

Connectivity webs mediate the unique biology of the mammalian brain. Yet, while cell circuit maps are increasingly available, knowledge of their underlying molecular networks remains limited. Here, we applied multi-dimensional biochemical fractionation with mass spectrometry and machine learning to survey endogenous macromolecules across the adult mouse brain. We defined a global "interactome" comprising over one thousand multi-protein complexes. These include ... [more]

Cell Syst Apr. 22, 2020; 10(4);333-350.e14 [Pubmed: 32325033]

Quantitative Score

0.938 [EPIC Score]

Throughput

High Throughput

Additional Notes

High confidence interactions had an EPIC Score >= 0.683

Curated By

BioGRID

Interaction Summary

ALK

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein tyrosine kinase activity [ISO]transmembrane receptor protein tyrosine kinase activity [ISO, ISS]

Gene Ontology Cellular Component

ERBB3

Gene Ontology Biological Process

Gene Ontology Molecular Function

growth factor binding [ISO]identical protein binding [ISO]neuregulin binding [ISO]neuregulin receptor activity [ISO]protein binding [IPI]protein heterodimerization activity [ISO]protein tyrosine kinase activator activity [ISO]protein tyrosine kinase activity [ISO]