PREY

PKS1

PHYTOCHROME KINASE SUBSTRATE 1, T17M13.12, T17M13_12, AT2G02950
phytochrome kinase substrate 1
GO Process (6)
GO Function (1)
GO Component (2)
Arabidopsis thaliana (Columbia)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

PKS1 plays a role in red-light-based positive phototropism in roots.

Molas ML, Kiss JZ

Aerial parts of plants curve towards the light (i.e. positive phototropism), and roots typically grow away from the light (i.e. negative phototropism). In addition, Arabidopsis roots exhibit positive phototropism relative to red light (RL), and this response is mediated by phytochromes A and B (phyA and phyB). Upon light stimulation, phyA and phyB interact with the phytochrome kinase substrate (PKS1) ... [more]

Plant Cell Environ. Jun. 01, 2008; 31(6);842-9 [Pubmed: 18266898]

Throughput

  • Low Throughput

Additional Notes

  • Pks1 suppresses the elongated root phenotype of phyB.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PHYB PKS1
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
268468
PHYB PKS1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID