Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

BraInMap Elucidates the Macromolecular Connectivity Landscape of Mammalian Brain.

Pourhaghighi R, Ash PEA, Phanse S, Goebels F, Hu LZM, Chen S, Zhang Y, Wierbowski SD, Boudeau S, Moutaoufik MT, Malty RH, Malolepsza E, Tsafou K, Nathan A, Cromar G, Guo H, Abdullatif AA, Apicco DJ, Becker LA, Gitler AD, Pulst SM, Youssef A, Hekman R, Havugimana PC, White CA, Blum BC, Ratti A, Bryant CD, Parkinson J, Lage K, Babu M, Yu H, Bader GD, Wolozin B, Emili A

Connectivity webs mediate the unique biology of the mammalian brain. Yet, while cell circuit maps are increasingly available, knowledge of their underlying molecular networks remains limited. Here, we applied multi-dimensional biochemical fractionation with mass spectrometry and machine learning to survey endogenous macromolecules across the adult mouse brain. We defined a global "interactome" comprising over one thousand multi-protein complexes. These include ... [more]

Cell Syst Apr. 22, 2020; 10(4);333-350.e14 [Pubmed: 32325033]

Quantitative Score

0.696 [EPIC Score]

Throughput

High Throughput

Additional Notes

High confidence interactions had an EPIC Score >= 0.683

Curated By

BioGRID

Interaction Summary

SYNGAP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

Rab GTPase activator activity [IDA]Ras GTPase activator activity [ISO]protein binding [IPI]protein kinase binding [ISO]

Gene Ontology Cellular Component

RALBP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity [ISO]ATPase activity, coupled to movement of substances [ISO]GTPase activator activity [IDA, ISO]Rac GTPase activator activity [IDA, ISO]Rac GTPase binding [ISO]Ral GTPase binding [IPI, ISO]protein binding [IPI]