BAIT

NIA2

ARABIDOPSIS NITRATE REDUCTASE 2, ATNR2, B29, CHL3, CHLORATE RESISTANT 3, F28L22.2, F28L22_2, NIA2-1, NITRATE REDUCTASE, NITRATE REDUCTASE 2, NR, NR2, AT1G37130
nitrate reductase [NADH] 2
GO Process (4)
GO Function (2)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

GRF13

GF 14 PI CHAIN, GF14 PI, T11I11.16, T11I11_16, general regulatory factor 13, AT1G78220
general regulatory factor 13
GO Process (0)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Ser-534 in the hinge 1 region of Arabidopsis nitrate reductase is conditionally required for binding of 14-3-3 proteins and in vitro inhibition.

Kanamaru K, Wang R, Su W, Crawford NM

14-3-3 proteins bind to the hinge 1 region of nitrate reductase (NR) and inhibit its activity. To determine which residues of NR are required for 14-3-3-inhibitory interactions, wild-type and mutant forms of Arabidopsis NR were examined in the yeast two-hybrid system and in vitro inhibition assays. NR fragments with or without hinge 1 were introduced into yeast with one of ... [more]

J. Biol. Chem. Feb. 12, 1999; 274(7);4160-5 [Pubmed: 9933611]

Throughput

  • Low Throughput

Curated By

  • BioGRID