AP1S2
Gene Ontology Biological Process
Gene Ontology Cellular Component
AP1G1
Gene Ontology Biological Process
- Golgi to lysosome transport [IMP]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- endosome to melanosome transport [IMP]
- melanosome organization [IC]
- membrane organization [TAS]
- positive regulation of natural killer cell degranulation [IMP]
- positive regulation of natural killer cell mediated cytotoxicity [IMP]
- post-Golgi vesicle-mediated transport [TAS]
- regulation of defense response to virus by virus [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- AP-type membrane coat adaptor complex [TAS]
- Golgi apparatus [IDA]
- Golgi membrane [TAS]
- clathrin-coated vesicle [IDA]
- clathrin-coated vesicle membrane [ISS]
- cytoplasm [IDA]
- cytoplasmic vesicle membrane [TAS]
- cytosol [TAS]
- intracellular membrane-bounded organelle [IDA]
- lysosomal membrane [TAS]
- membrane [IDA]
- recycling endosome [IDA]
- trans-Golgi network membrane [TAS]
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
An interactome perturbation framework prioritizes damaging missense mutations for developmental disorders.
Identifying disease-associated missense mutations remains a challenge, especially in large-scale sequencing studies. Here we establish an experimentally and computationally integrated approach to investigate the functional impact of missense mutations in the context of the human interactome network and test our approach by analyzing ~2,000 de novo missense mutations found in autism subjects and their unaffected siblings. Interaction-disrupting de novo missense ... [more]
Throughput
- High Throughput
Additional Notes
- this interaction found to remain intact in at least one case as part of an assessment of the effect on protein-protein interactions of autism-spectrum disorder mutations in the bait proteins
- this interaction was found to remain intact in the case of at least one bait protein mutation as part of an assessment of the effect on protein-protein interactions of autism-spectrum disorder mutations in the bait proteins
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AP1S2 AP1G1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 1443063 | |
| AP1S2 AP1G1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 2218180 | |
| AP1S2 AP1G1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3063904 | |
| AP1G1 AP1S2 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | - | BioGRID | 3789245 | |
| AP1G1 AP1S2 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.888 | BioGRID | 741680 | |
| AP1S2 AP1G1 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.9803 | BioGRID | 1258619 | |
| AP1G1 AP1S2 | Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071). | High | - | BioGRID | 3791531 | |
| AP1G1 AP1S2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
| AP1S2 AP1G1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID