Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

An interactome perturbation framework prioritizes damaging missense mutations for developmental disorders.

Chen S, Fragoza R, Klei L, Liu Y, Wang J, Roeder K, Devlin B, Yu H

Identifying disease-associated missense mutations remains a challenge, especially in large-scale sequencing studies. Here we establish an experimentally and computationally integrated approach to investigate the functional impact of missense mutations in the context of the human interactome network and test our approach by analyzing ~2,000 de novo missense mutations found in autism subjects and their unaffected siblings. Interaction-disrupting de novo missense ... [more]

Nat. Genet. Dec. 01, 2017; 50(7);1032-1040 [Pubmed: 29892012]

Throughput

High Throughput

Additional Notes

this interaction found to remain intact in at least one case as part of an assessment of the effect on protein-protein interactions of autism-spectrum disorder mutations in the bait proteins
this interaction was found to remain intact in the case of at least one bait protein mutation as part of an assessment of the effect on protein-protein interactions of autism-spectrum disorder mutations in the bait proteins

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HOMER3 ABI2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2017)	High	0.9825	BioGRID	2244241
HOMER3 ABI2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9525	BioGRID	3140295
HOMER3 ABI2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Luck K (2020)	High	-	BioGRID	2696640
HOMER3 ABI2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Rolland T (2014)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ABI2

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [TAS]Rac GTPase binding [IDA]SH3 domain binding [IPI, TAS]cytoskeletal adaptor activity [TAS]kinase binding [NAS]proline-rich region binding [IPI]protein binding [IPI]protein complex binding [IDA]ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

HOMER3

Gene Ontology Biological Process

Gene Ontology Molecular Function

G-protein coupled glutamate receptor binding [IBA]protein binding [IPI]