BAIT

CCDC120

JM11
coiled-coil domain containing 120
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

CYTH2

ARNO, CTS18, CTS18.1, PSCD2, PSCD2L, SEC7L, Sec7p-L, Sec7p-like
cytohesin 2
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

An interactome perturbation framework prioritizes damaging missense mutations for developmental disorders.

Chen S, Fragoza R, Klei L, Liu Y, Wang J, Roeder K, Devlin B, Yu H

Identifying disease-associated missense mutations remains a challenge, especially in large-scale sequencing studies. Here we establish an experimentally and computationally integrated approach to investigate the functional impact of missense mutations in the context of the human interactome network and test our approach by analyzing ~2,000 de novo missense mutations found in autism subjects and their unaffected siblings. Interaction-disrupting de novo missense ... [more]

Nat. Genet. Dec. 01, 2017; 50(7);1032-1040 [Pubmed: 29892012]

Throughput

  • High Throughput

Additional Notes

  • this interaction was found to remain intact in the case of at least one bait protein mutation as part of an assessment of the effect on protein-protein interactions of autism-spectrum disorder mutations in the bait proteins

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CCDC120 CYTH2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
2220240
CCDC120 CYTH2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
3075881
CYTH2 CCDC120
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
2709993
CYTH2 CCDC120
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
CYTH2 CCDC120
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID