BAIT

PSME3

HEL-S-283, Ki, PA28-gamma, PA28G, PA28gamma, REG-GAMMA
proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
GO Process (24)
GO Function (4)
GO Component (6)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

REG? Controls Hippo Signaling and Reciprocal NF-?B-YAP Regulation to Promote Colon Cancer.

Wang Q, Gao X, Yu T, Yuan L, Dai J, Wang W, Chen G, Jiao C, Zhou W, Huang Q, Cui L, Zhang P, Moses RE, Yang J, Chen F, Fu J, Xiao J, Li L, Dang Y, Li X

Purpose: Colorectal cancer is one of the most commonly diagnosed cancers closely associated with inflammation and hyperactive growth. We previously demonstrated a regulatory circuit between the proteasome activator REG? and NF-kappaB (NF-?B) during colon inflammation, known to be important in the development of colitis-associated cancer as well as sporadic colorectal cancer. How the inflammatory microenvironment affects the Hippo pathway during ... [more]

Clin. Cancer Res. Dec. 15, 2017; 24(8);2015-2025 [Pubmed: 29437787]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LATS1 PSME3
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID