Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

KAP1 facilitates reinstatement of heterochromatin after DNA replication.

Jang SM, Kauzlaric A, Quivy JP, Pontis J, Rauwel B, Coluccio A, Offner S, Duc J, Turelli P, Almouzni G, Trono D

During cell division, maintenance of chromatin features from the parental genome requires their proper establishment on its newly synthetized copy. The loss of epigenetic marks within heterochromatin, typically enriched in repetitive elements, endangers genome stability and permits chromosomal rearrangements via recombination. However, how histone modifications associated with heterochromatin are maintained across mitosis remains poorly understood. KAP1 is known to act ... [more]

Nucleic Acids Res. Dec. 28, 2017; 46(17);8788-8802 [Pubmed: 29955894]

Throughput

  • High Throughput

Additional Notes

  • hit proteins identified by MS (mass spectrometry) in KAP1 IP (immunoprecipitation), with T test p values < 0.01

Curated By

  • BioGRID