An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Quantitative interaction proteomics of neurodegenerative disease proteins.

Hosp F, Vossfeldt H, Heinig M, Vasiljevic D, Arumughan A, Wyler E, , Landthaler M, Hubner N, Wanker EE, Lannfelt L, Ingelsson M, Lalowski M, Voigt A, Selbach M

Several proteins have been linked to neurodegenerative disorders (NDDs), but their molecular function is not completely understood. Here, we used quantitative interaction proteomics to identify binding partners of Amyloid beta precursor protein (APP) and Presenilin-1 (PSEN1) for Alzheimer's disease (AD), Huntingtin (HTT) for Huntington's disease, Parkin (PARK2) for Parkinson's disease, and Ataxin-1 (ATXN1) for spinocerebellar ataxia type 1. Our network ... [more]

Cell Rep May. 19, 2015; 11(7);1134-46 [Pubmed: 25959826]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

ATXN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]poly(G) binding [IDA]poly(U) RNA binding [IDA]protein C-terminus binding [IPI]protein binding [IPI]protein self-association [IDA]

Gene Ontology Cellular Component

MLF2