EIF4E
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
FMR1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell body [IDA]
- cytoplasm [ISO]
- cytoplasmic ribonucleoprotein granule [ISO]
- cytoplasmic stress granule [ISO]
- dendrite [IDA, ISO]
- dendritic shaft [IDA]
- dendritic spine [IDA]
- mRNA cap binding complex [ISO, ISS]
- membrane [ISO]
- microtubule [IDA]
- neuron projection [IDA]
- neuronal ribonucleoprotein granule [IDA, ISO]
- nucleus [IBA, ISO]
- polysome [IDA]
- synapse [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
FMRP recruitment of β-catenin to the translation pre-initiation complex represses translation.
Canonical Wnt/β-catenin signaling is an essential regulator of various cellular functions throughout development and adulthood. Aberrant Wnt/β-catenin signaling also contributes to various pathologies including cancer, necessitating an understanding of cell context-dependent mechanisms regulating this pathway. Since protein-protein interactions underpin β-catenin function and localization, we sought to identify novel β-catenin interacting partners by affinity purification coupled with tandem mass spectrometry in ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID