BAIT

COP8

ATS4, CONSTITUTIVE PHOTOMORPHOGENIC 14, CONSTITUTIVE PHOTOMORPHOGENIC 8, COP14, COP9 SIGNALOSOME SUBUNIT 4, CSN4, EMB134, EMBRYO DEFECTIVE 134, FUS4, FUS8, FUSCA 4, FUSCA 8, MBD2.17, MBD2_17, AT5G42970
COP9 signalosome complex subunit 4
GO Process (3)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

CSN6A

COP9 signalosome subunit 6A, MXK23.2, MXK23_2, AT5G56280
COP9 signalosome complex subunit 6a
GO Process (5)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The COP9 signalosome interacts physically with SCF COI1 and modulates jasmonate responses.

Feng S, Ma L, Wang X, Xie D, Dinesh-Kumar SP, Wei N, Deng XW

The COP9 signalosome (CSN) is an evolutionarily conserved, nucleus-enriched multiprotein complex. CSN plays roles in photomorphogenesis, auxin response, and floral organ formation, possibly via the regulation of ubiquitin-proteasome-mediated protein degradation. COI1 encodes an F-box protein, which is a subunit of SCF(COI1) E3 ubiquitin ligase, and is required for jasmonate (JA) responses. Here, we demonstrate using coimmunoprecipitation and gel-filtration analyses that ... [more]

Plant Cell May. 01, 2003; 15(5);1083-94 [Pubmed: 12724535]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CSN6A COP8
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.8867BioGRID
2912779
COP8 CSN6A
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID